One dinuclear complex [Cu2Cl2(HEMDP)2]·THF 1 (H2EMDP = 2-[(2-hydroxy- ethylimino)-methyl]-4,6-diiodo-phenol, THF = tetrahydrofuran) has been designed and synthesized by H2EMDP with CuCl2·2H2O in THF solvent. Complex 1 was characterized by UV, IR, ESI-MS, and elemental analyses. 1 was characterized by X-ray crystallography. The crystal belongs to the monoclinic system, space group C2/c with a = 27.886(3), b = 8.6890(12), c = 26.698(2) Å, β = 105.521(2)º, V = 6233.1(12) Å3, Z = 8, Mr= 1102.01, m = 5.533 mm-1, Dc = 2.349 Mg/m3, F(000) = 4112, the final R = 0.0486 and wR = 0.1175 (I > 2σ(I)). In addition, further investigation revealed that the central copper(II) atom in the complex is five-coordinated by one nitrogen atom and two oxygen atoms from H2EMDP and two chlorine atoms from chlorid. The 3,5-diiodosalicylaldehyde Schiff base acts as a tridentate ligand. H2EMDP and 1 were assayed for antibacterial (B. subtilis, S. aureus, S. faecalis, P. aeruginosa, E. coli and E. cloacae) activities by MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl trtrazolium bromide) method. 1 showed the most favorable antimicrobial activity with MICs of 3.125, 6.25, 6.25, 12.5, 3.125 and 6.25 μg/mL against B. subtilis, S. aureus, S. faecalis, P. aeruginosa, E. coli and E.cloacae, respectively.